Figure 1
From: Incorporation of mammalian actin into microfilaments in plant cell nucleus
Labeling of microfilaments in onion bulb scale epidermis cells. Labeling of microfilaments in onion bulb scale epidermis cells with rhodamine-phalloidin by freeze shattering (A-B, E-F) and enzyme-mediated permeabilization (C-D), and with GFP-talin (H-G). Sum projections of 90 optical slices (A, C, E, G), and single optical slices through the central region of nuclei (B, D, F, H). Arrows, the spot-like F-actin structures in the nuclei; arrowheads, the nuclear basket. Bars, 20 μm.