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Table 1 Frequencies of gametophyte phenotypes after bombardment with various plasmids.

From: A systemic gene silencing method suitable for high throughput, reverse genetic analyses of gene function in fern gametophytes

Plasmids introduced % colorless & GUS positivea,b % colorless & GUS negativea % green & GUS positivea nc
35S:irintCrChl+pFF19G 88.8 (+/-1.36)d 6.8 (+/-0.46)d 4 (+/- 1.41)d 1263
irintCrChl+pFF19G 81.7 (+/-0.87) 8.3 (+/-1.20) 10 (+/-1.53) 775
35S:intantiCrChl+pFF19G 61.0 (+/-1.73) 11.0 (+/-1.73) 28.0 (+/-3.21) 564
35S:antiCrChl+pFF19G 12.0 (+/-1.53) 0 88.0 (+/-1.53) 724
antiCrChl+pFF19G 0.7 (+/-0.33) 0 99.3 (+/-0.33) 479
35S:senseCrChlint+pFF19G 37.7 (+/-1.12) 6.3 (+/-0.19) 56 (+/-1.00) 559
35S:senseCrChl+pFF19G 2.3 (+/-0.88) 0 97.7 (+/-0.88) 761
senseCrChl+pFF19G 1.7 (+/-0.33) 0 98.7 (+/-0.42) 677
CrChl cDNA+pFF19G 13.7 (+/-0.88) 0 86.0 (+/-1.16) 563
Δ35SpFF19G+ 48.5 (+/-6.36) 51.0 (+/-5.66) 0.5 (+/-0.71) 470
35SinintCrChl     
pFF19G 0 0 100 600
  % with lesions & GUS positivea,b % with lesions & GUS negativea % without lesions & GUS positive nc
CrUrod cDNA+pFF19G 61.0 (+/-0.71) 20.2 (+/-0.69) 18.9 (+/-0.43) 774
35S:intantiCrUrod+pFF19G 55.7 (+/-1.40) 13.5 (+/-1.61) 31.1 (+/- 2.25) 472
  1. aAll gametophytes were stained 7 days after shooting; each percent represents the average of three replicates; standard error is given in parentheses. bthe differences between treatments for all pairwise comparisons are statistically significant (z>3.4, P < 0.0003) with the exception of 35S:senseCrChl+pFF19G and senseCrChl+pFF19G. ctotal number of gametophytes displaying a phenotype and scored. dthe average of 5 replicates.