Figure 5

Long-term ¹⁰⁹Cd accumulation in roots of durum wheat seedlings. Time-course of ¹⁰⁹Cd accumulation in intact roots (A) of high (TL-H) and low (TL-L) Cd-accumulating isolines of durum wheat. Roots of 6-d old seedlings were exposed for up to 60 h (solutions changed every 12 h) in 15 mL of complete nutrient solution containing 50 pM ¹⁰⁹Cd and 0.5 μM ⁶⁵Zn. Desorbable ¹⁰⁹Cd (B) was removed following treatment by a 30 min (2°C) wash in non-radiolabelled nutrient solution containing 50 μM DTPA. Inset shows desorbable ¹⁰⁹Cd expressed as a percentage of total ¹⁰⁹Cd accumulation in the roots. Means and standard errors of 7 replicates (3 plants per replicate) are plotted.