Table 2 Substrate specificity of the recombinant F6H Both rF6Hb and rF6Hy were purified on Ni-NTA resin then Superose 12, and fractions were assayed with the indicated substrates, using the direct enzyme assay.
aNo F6H activity was detected with kaempferol, quercetin, myricetin, naringenin, eriodyctyol, apigenin or luteolin when used as substrate, regardless of concentration. Rhamnetin (7-O-methylquercetin, 17% at 50 μM), tamarixetin (4'-O-methylquercetin, 13 % at 50 μM) and isorhamnetin (3'-O-methylquercetin, 4 % at 50 μM)
bEstimated as 0.10 pkat/mg for 100% activity and 5 μM substrate.
cEstimated as 0.19 pkat/mg for 100% activity and 5 μM substrate.