Table 1 Specific activity of recombinant F6H proteinsa
From: Molecular characterization and functional expression of flavonol 6-hydroxylase
Step | Specific Activity (pkat/mg) |
|---|---|
rF6Hb | |
Cell Lysate | 0 |
Ni-NTA | 0.091 |
Ni-NTA (30°C) | 0.087 |
Ni-NTA + Superose 12 | 0.103 |
Superose 12 | 0.075 |
Cleavage of (His)6-tag after Ni-NTA | 0.044 |
rF6Hy (intracellular) | |
Cell Lysate | 0 |
Ni-NTA | 0.17 |
Ni-NTA + Superdex 75 | 0.21 |
Superdex 75 | 0.087 |
rF6Hys (secreted) | |
(NH4)2SO4 (35–70%) | 0 |
(NH4)2SO4 + Ni-NTA | 0.11 |
(NH4)2SO4 + Ni-NTA + Superdex 75 | 0.08 |
Superdex 75 | 0.04 |