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Table 1 Effect of light on AsAt content in source leaves, tuberising stolons and exudates

From: Long-distance transport of L-ascorbic acid in potato

Tissue

Cycle

AsAt in tissue (mg/100 gFW)

AsAt in exudate (peak area mAUt)

Leaf

Light

42.5 ± 3.1

1.1 ± 0.1

 

Dark

19.1 ± 3.0

0.6 ± 0.05

Tuberising stolon

Light

4.1 ± 0.3

7.9 ± 0.8

 

Dark

4.5 ± 0.4

1.7 ± 0.9

  1. Glasshouse grown plants were transferred to off-phased controlled environment chambers 14 days prior to the start of the experiment. Environment chambers were on a 10 h dark – 14 h light cycle (see Fig. 4). At 12:00 h source leaves and tuberising stolons were removed from 3 plants and a sub-sample used for tissue AsAt quantification. For the determination of AsAt in leaf phloem exudates, petioles were re-cut under water and placed into EDTA or CaCl2 exudation buffer for 90 min in a prehumidified chamber in the dark. For determination of stolon phloem exudates the cut end of the stolon attached to the plant was re-cut under water and placed in the appropriate exudation solution. Values are represented as mean ± SE, n = 6. mAUt = milli absorbance units (λ245 nm) × time.