Figure 6
Effect of precursor supply to source leaves on AsA t content in sink tissues. Terminal leaflets of all leaves of the lower four nodes of each stem of four potato plants were incubated with 500 μl 20 mM MES pH 5.5, 2 mM CaCl2 alone (control) or containing 25 mM L-GalL for 24 h. At the end of incubation the indicated tissues were removed from plants, snap frozen in liquid nitrogen and lyophilised. After lyophilisation, tissues were powdered and extracted in 5% MPA, 5 mM TCEP (19:1 v/w) prior to estimation of the AsA content in each tissue by HPLC. Values are presented as means ± SE, n = 4.