Figure 5From: Cloning and expression analysis of cDNAs corresponding to genes activated in cucumber showing systemic acquired resistance after BTH treatmentTime course of mRNA accumulation in cucumber leaves after BTH treatment (A) or C. lagenarium inoculation (B). A: Total RNA was extracted from cucumber leaves at various time after application of 10 μM BTH or water (mock treatment). RNA (15 μg) from leaves 1 and 2 (treated) and leaves 3 and 4 (untreated) was fractionated by electrophoresis on agarose gels. Identical Northern blots were probed with 32P-labeled PR-8, CPR and CGT cDNAs. B: Total RNA was extracted from cucumber leaves at various time after C. lagenarium inoculation. RNA (15 μg) from untreated leaves (UT), leaves 1 (infected) and leaves 2, 3 and 4 (non infected) was fractionated by electrophoresis on agarose gels. Identical Northern blots were probed with 32P-labeled PR-8, CPR and CGT cDNAs. Equal loading of RNA was confirmed by ethidium bromide (Et Br) staining. A representative gel is shown.Back to article page