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Table 1 Identity and percent composition of Se species in shoot tissue of SMT over-producing A. thaliana line smt2-9.

From: Production of Se-methylselenocysteine in transgenic plants expressing selenocysteine methyltransferase

Se species Peak # tR (min) m/z μmol g -1 dry weight % of extractable shoot Se
1Selenite nd nd nd 0.16 3
2MeSeCys 1 4.75 167 (M+H-NH3)+ 0.71 12
2MeSeCys 2 6.25 167 (M+H-NH3)+ 1.68 31
2Unidentified 3 8.75 nd 0.27 5
3 γGluMeSeCys 4 7.5 313 (M+H)+ 1.16 26
3Unidentified 5 10.5 nd 0.37 6
3Unaccounted - - - - 17
  1. 1Calculated using XAS data. 2Mobile phase water-methanol (99:1, v/v) 0.1% HFBA. 3Mobile phase water-methanol (99:1, v/v) 0.1% TFA. HPLC fractions (0.5 – 1.0 ml) were collected and analyzed for total Se by ICP-MS. Replicate chromatography was also performed using HPLC-ESI-MS to confirm the identity of the compounds in the Se containing peaks. The various Se compounds were quantified based on their Se content. Chromatography and mass spectroscopy data shown in Figure 2.