|
Se species
|
Peak #
|
tR (min)
|
m/z
|
μmol g
-1
dry weight
|
% of extractable shoot Se
|
|---|
|
1Selenite
|
nd
|
nd
|
nd
|
0.16
|
3
|
|
2MeSeCys
|
1
|
4.75
|
167 (M+H-NH3)+
|
0.71
|
12
|
|
2MeSeCys
|
2
|
6.25
|
167 (M+H-NH3)+
|
1.68
|
31
|
|
2Unidentified
|
3
|
8.75
|
nd
|
0.27
|
5
|
|
3 γGluMeSeCys
|
4
|
7.5
|
313 (M+H)+
|
1.16
|
26
|
|
3Unidentified
|
5
|
10.5
|
nd
|
0.37
|
6
|
|
3Unaccounted
|
-
|
-
|
-
|
-
|
17
|
- 1Calculated using XAS data. 2Mobile phase water-methanol (99:1, v/v) 0.1% HFBA. 3Mobile phase water-methanol (99:1, v/v) 0.1% TFA. HPLC fractions (0.5 – 1.0 ml) were collected and analyzed for total Se by ICP-MS. Replicate chromatography was also performed using HPLC-ESI-MS to confirm the identity of the compounds in the Se containing peaks. The various Se compounds were quantified based on their Se content. Chromatography and mass spectroscopy data shown in Figure 2.
