Impact of SMT protein levels on accumulation of MeSeCys and total Se. (A) Relative SMT protein accumulation. Relative SMT protein levels were determined from digitized immunoblots, and represent the average band intensity (± SE) from 12 – 18 individual plants for each line. (B) Concentration of MeSeCys in transgenic plants. MeSeCys was quantified using HPLC (AccQ Tag amino acid analysis system) and its identify confirmed using MALDI-MS. Data represents the average (± SE) MeSeCys concentrations in 11 – 18 individual plants for each line. (C) Concentration of total Se in transgenic plants. Total Se was quantified by ICP-MS, and data represents the average (± SE) of 7 – 13 individual plants for each line.