Table 5 Characteristics of 41 SSR loci produced by 21 primer pairs showing multilocus segregation. Are indicated: total number of alleles, number of polymorphic alleles, expected genotypes of the parents as determined by TetraploidMap software in alfalfa F1 mapping population from Mercedes4.11 Ć Magali2 (the non segregating bands in F1 were not considered), double reduction coefficient (Ī±) and its statistical significant (LR). '0' indicated null allele
Locus | Total number of alleles | Number of polymorphic alleles | Genotype | of | Double | reduction | Remark |
|---|---|---|---|---|---|---|---|
| Ā | Ā | Ā | Mercedes4.11 | Magali2 | Ī± | LR test 1 | Ā |
AI974357-1 | 4 | 4 | CCD0 | AB00 | 0.00 | 0.00 | Ā |
AI974357-2 | 2 | 2 | FF00 | EE00 | 0.04 | 0.19 | Ā |
B21E13-1 | 1 | 1 | A000 | A000 | 0.13 | 0.90 | Ā |
B21E13-2 | 4 | 4 | CDE0 | BCCD | 0.00 | 0.00 | Ā |
B21E13-3 | 1 | 1 | F000 | 0000 | 0.00 | 0.00 | Ā |
MTIC12-1 | 4 | 4 | ABCC | ABCD | 0.00 | 0.00 | Ā |
MTIC12-2 | 2 | 2 | E000 | EF00 | 0.02 | 0.02 | Ā |
MTIC19-1 | 3 | 3 | AA00 | AABC | 0.00 | 0.00 | Ā |
MTIC19-2 | 2 | 2 | DE00 | 0000 | 0.07 | 0.55 | Ā |
MTIC19-3 | 1 | 1 | F000 | 0000 | 0.04 | 0.06 | Ā |
MTIC48-1 | 4 | 4 | A000 | BCD0 | 0.00 | 0.00 | Ā |
MTIC48-2 | 1 | 1 | EE00 | 0000 | 0.14 | 2.17 | Ā |
MTIC51-1 | 3 | 3 | ABC0 | ABC0 | 0.09 | 39.05 | Ā |
MTIC51-2 | 2 | 2 | DE00 | 0000 | 0.04 | 0.19 | Ā |
MTIC77-1 | 2 | 2 | 0000 | AB00 | 0.02 | 0.05 | Ā |
MTIC77-2 | 4 | 4 | CDEF | CDDE | 0.00 | 0.00 | Ā |
MTIC77-3 | 1 | 1 | G000 | 0000 | 0.07 | 0.22 | Ā |
MTIC82 | 3 | 1 | C000 | 0000 | 0.16 | 2.59 | Alleles A and B monomorphic, present in both parents |
MTIC84-1 | 1 | 1 | 0000 | A000 | 0.00 | 0.00 | Ā |
MTIC84-2 | 4 | 4 | BCE0 | DD00 | 0.00 | 0.00 | Ā |
MTIC84-3 | 1 | 1 | 0000 | F000 | 0.09 | 0.30 | Ā |
MTIC93 | 3 | 1 | C000 | 0000 | 0.15 | 0.89 | Alleles A and B monomorphic, present in both parents |
MTIC107 | 3 | 2 | AA00 | AAC0 | 0.05 | 3.78 | Allele B monomorphic, present in both parents |
MTIC134-1 | 5 | 5 | BCE0 | AD00 | 0.04 | 63.0 | Ā |
MTIC134-2 | 2 | 2 | F000 | G000 | 0.00 | 0.00 | Ā |
MTIC135-1 | 2 | 2 | B000 | AB00 | 0.00 | 0.00 | Ā |
MTIC135-2 | 2 | 2 | CD00 or CDD0 | CDD0 or CD00 | 0.12 | 0.77 | Ā |
MTIC135-3 | 1 | 1 | E000 | 0000 | 0.00 | 0.00 | Ā |
MTIC183-1 | 5 | 5 | ACDE | BC00 | 0.02 | 43.74 | Ā |
MTIC183-2 | 1 | 1 | 0000 | F000 | 0.00 | 0.00 | Ā |
MTIC185 | 3 | 2 | B000 | BC00 | 0.16 | 0.12 | Allele A monomorphic, present in both parents |
MTIC188-1 | 3 | 3 | B000 | AC00 | 0.06 | 0.63 | Ā |
MTIC188-2 | 4 | 4 | DEG0 | DF00 | 0.00 | 0.00 | Ā |
MTIC238-1 | 3 | 3 | AACC | ABC0 | 0.16 | 57.67 | Ā |
MTIC238-2 | 3 | 3 | DEF0 | FF00 | 0.03 | 5.18 | Ā |
MTIC250-1 | 2 | 2 | B000 | AABB | 0.00 | 0.00 | Ā |
MTIC250-2 | 2 | 2 | 0000 | CD00 | 0.10 | 1.28 | Ā |
MTIC258 | 4 | 2 | 0000 | AD00 | 0.16 | 22.9 | Alleles B and C monomorphic, present in both parents |
MTIC304 | 4 | 3 | BBDD | ABDO | 0.00 | 0.00 | Allele C monomorphic, present in both parents |
MTLEC2A-1 | 2 | 2 | 0000 | AB00 | 0.00 | 0.00 | Ā |
MTLEC2A-2 | 2 | 1 | 0000 | D000 | 0.00 | 0.00 | Allele C monomorphic, present in both parents |