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Table 4 Characteristics of 63 SSR loci with tetrasomic inheritance. Are indicated: total number of alleles, number of polymorphic alleles, expected genotypes of the parents as determined by TetraploidMap software in alfalfa F1 mapping population from Mercedes4.11 × Magali2 (the non segregating bands in F1 were not considered), double reduction coefficient (α) and its statistical significant (LR). '0' indicated null allele. The letter indicating the allele (A-G) was attributed from the lowest to the highest molecular weight.

From: Construction of two genetic linkage maps in cultivated tetraploid alfalfa (Medicago sativa) using microsatellite and AFLP markers

Locus Total number of alleles Number of polymorphic alleles Genotype of Double reduction Remark
    Mercedes4.11 Magali2 α LR test 1  
Loci with non significant double reduction
FMT13 3 3 ABBC AABB 0.00 0.00  
MAW208206 1 1 0000 AA00 0.02 0.04  
MAA660456 4 4 ABBC ABCD 0.00 0.00  
MAL368684 3 3 ABC0 C000 0.00 0.00  
E318681 2 1 0000 B000 0.13 0.59 Allele A monomorphic, present in both parents
MTIC27 2 2 AAB0 AA00 0.14 1.27  
MTIC35 2 1 B000 or BB00 BB00 or B000 0.04 0.17 Allele A monomorphic, present in both parents
MTIC58 2 1 0000 A000 0.07 0.22 Allele B monomorphic, present in both parents
MTIC64 3 2 A000 AC00 0.03 0.10 Allele B monomorphic, present in both parents
MTIC79 3 2 AB00 0000 0.00 0.00 Allele C monomorphic, present in both parents
MTIC90 1 1 A000 0000 0.02 0.02  
MTIC95 3 3 AACC AABC 0.00 0.00  
MTIC103 4 4 ACDD BDD0 0.00 0.00  
MTIC124 3 3 AACC AB00 0.05 0.63  
MTIC131 4 4 BCD0 ABD0 0.00 0.00  
MTIC145 3 3 0000 ABC0 0.00 0.00  
MTIC169 3 3 BC00 AA00 0.00 0.00  
MTIC189 5 5 ACD0 ABDE 0.00 0.00  
MTIC210 3 3 AABC AABC 0.05 0.53  
MTIC232 2 1 B000 0000 0.00 0.00 Allele A monomorphic, present in both parents
MTIC237 2 1 B000 0000 0.12 0.61 Allele A monomorphic, present in both parents
MTIC247 2 2 AAB0 AA00 0.03 0.07  
MTIC248 3 3 BC00 A000 0.16 0.76  
MTIC272 3 2 CC00 BC00 0.01 0.02 Allele A monomorphic, present in both parents
MTIC289 4 3 0000 ACD0 0.00 0.00 Allele B monomorphic present in both parents
MTIC299 3 3 CC00 ABC0 0.00 0.00  
MTIC314 2 1 0000 B000 0.00 0.00 Allele A monomorphic, present in both parents
MTIC318 2 1 B000 0000 0.15 0.91 Allele A monomorphic, present in both parents
MTIC332 3 3 AAB0 ABC0 0.00 0.00  
MTIC338 3 2 C000 BBCC 0.03 0.26 Allele A monomorphic, present in Mercedes4.11
MTIC339 3 2 BC00 B000 0.00 0.00 Allele A monomorphic, present in both parents
MTIC343 4 4 BBCD ABDD 0.00 0.00  
MTIC345 5 5 BCDE AADD 0.00 0.00  
MTIC347 3 2 AA00 C000 0.05 0.40 Allele B monomorphic, present in both parents
MTIC354 4 4 ABCD AABC 0.00 0.00  
MTIC356 2 1 0000 A000 0.16 2.13 Allele B monomorphic, present in both parents
MTIC365 4 4 ABCD ABCC 0.00 0.00  
MTIC441 1 1 A000 0000 0.00 0.00  
MTIC446 3 2 B000 C000 0.06 0.32 Allele A monomorphic, present in both parents
MTIC451 5 5 ABCD ABCE 0.00 0.00  
MTIC470 2 1 A000 0000 0.00 0.00 Allele B monomorphic, present in both parents
MTIC471 2 2 AAB0 A000 0.00 0.00  
MTIC475 2 1 0000 B000 0.00 0.00 Allele A monomorphic, present in both parents
Loci with significant double reduction
Afct32 6 6 BCDF ABE0 0.01 77.5  
Afct45 4 4 ABBD AAC0 0.01 24.75  
B14B03 3 3 CC00 AB00 0.02 49.09  
MAA660870 5 5 ACDE ABE0 0.04 46.20  
MAL369471 3 3 AABC AA00 0.07 5.97  
MTR58 4 4 AAC0 BD00 0.01 98.04  
ENOD20 6 6 ADEF BCE0 0.01 3.85  
MTIC7 2 2 AB00 BB00 0.04 20.69  
MTIC14 3 2 BC00 BB00 0.16 4.82 A monomorphic, present in both parents
MTIC94 2 1 0000 AA00 0.16 5.09 B monomorphic, present in both parents
MTIC153 4 4 ABC0 BCD0 0.15 39.4  
MTIC230 4 4 BCD0 AD00 0.16 7.69  
MTIC233 3 3 BC00 A000 0.04 142.2  
MTIC249 2 2 BB00 AB00 0.08 61.48  
MTIC251 4 4 BC00 AAD0 0.07 78.00  
MTIC273 3 3 AAB0 ABC0 0.00 6.71  
MTIC326 4 3 D000 AC00 0.14 66.21 B monomorphic, present in both parents
MTIC331 2 1 0000 BB00 0.03 49.70 A monomorphic, present in both parents
MTIC432 5 5 ABC0 ACDE 0.01 46.20  
MTIC447 3 2 AC00 0000 0.16 50.99 B monomorphic, present in both parents
  1. 1 A LR test lower than a χ2 with 1 df (3.84) is not significant at P = 0.05