Figure 5From: Fusion to GFP blocks intercellular trafficking of the sucrose transporter SUT1 leading to accumulation in companion cellsYeast heterologous expression of SUT1-GFP. The functionality of the SUT1 in the SUT1-GFP fusion is shown by complementation of a yeast strain deficient in sucrose uptake mechanism. SUSY7 yeast strain was transformed with the plasmid containing the fusion LeSUT1-GFP under the Adh1 promoter and the empty vector (112A1NE). A similar amount of cells were plated on 2% sucrose or 2% glucose containing media.Back to article page