Figure 3

GFP Localization in RolC-GFP transgenic plants. GFP fluorescence was detected on longitudinal hand sections of transgenic tobacco petiole under CLSM and co-stained with DAPI and aniline blue. A) All constructs were prepared in a modified pGPTV-HPT under the control of the CC-specific, RolC, promoter. The asterisk indicates the position of the start codon. T, nos terminator. B) RolC-GFP petiole, C) RolC-GFP-3'-UTR petiole, D) major vein of mature leaf of RolC-GFP transgenic plant, E) sink leaf (1 cm long) of RolC-GFP transgenic plant, F) leaf petiole of a sink leaf of RolC-GFP transgenic plant, G) Stem of RolC-GFP transgenic plant, H) Veins of an unopened flower of RolC-GFP transgenic plant. I) Untransformed wild type tobacco leaf petiole visualized with the average PMT level used for the above images. CC, companion cell; SE, sieve element; sp, sieve plate; n, nucleus. Bar equals to 10 μm.