Figure 5
Effect of exogenous substrates on AsA coxntent of celery tissues. Isolated phloem strands or storage parenchyma discs were incubated for 18 h in 20 mM MES pH 5.5, 300 mM mannitol, 5 mM MgCl2, 2 mM KCl, 1 mM CaCl2, 1 mM CaSO4 (Daie, 1987) and 25 mM of the appropriate substrate. AsA was extracted and total AsA quantified by HPLC after reduction of DHA with 5 mM TCEP. Data represent mean ± S.E., n = 3.