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Table 2 PCR characterization of bfl1 mutant and its putative revertants (PR)

From: Ds tagging of BRANCHED FLORETLESS 1 (BFL1) that mediates the transition from spikelet to floret meristem in rice (Oryza sativaL)

Plant Ac PCR Ds PCR PCR1a PCR2a PCR3a Genotype of Ds insertion Phenotype
bfl1 + + - + + Ds/Ds Mutant
PR1 + - - + - Ds/Ds Mutant
PR2 + + + - - +/+ Normal
PR3 + + - + + Ds/Ds Mutant
PR4 + + - + + Ds/Ds Mutant
PR5 - + + - - +/+ Normal
PR6 + + - + + Ds/Ds Mutant
PR7 + + + + + Ds/+ Normal
PR8 + + - + + Ds/Ds Mutant
PR9 + + - + + Ds/Ds Mutant
PR10 - + - + + Ds/Ds Mutant
PR12 ND + + - - +/+ Normal
WT - - + - - +/+ Normal
  1. a): PCR1, PCR2 and PCR3 were done with primers LW1125_For and LW1125_Rev, LW1125_Rev and Ds5_112-, and LW1125_For and Ds3_6587+, respectively.