Figure 5
A & B. Southern analysis of T0 and T1 progeny of Triticum durum cv. PDW215 transformed with HVA1 gene (pBY520) by particle bombardment using mature embryo derived calli as explants. The numerals on the top of the lanes indicate the designation of progeny plants. Hybridization was performed with 2 kb HVA1 coding region-pin2 terminator fragment (2 kb fragment obtained after digestion of pBY520 with HindIII and PstI). UD: undigested, H: HindIII.