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Figure 4 | BMC Plant Biology

Figure 4

From: Genetic transformation of Indian bread (T. aestivum) and pasta (T. durum) wheat by particle bombardment of mature embryo-derived calli

Figure 4

A. Southern analysis of some T0 transformants of Triticum aestivum cv. CPAN1676 cotransformed with gus (pAct1-F) and bar (pDM302) by particle bombardment using mature embryo-derived calli. The genomic DNA of control and putative transformants (identities mentioned above) were digested with XhoI. Other details were similar to that described above. B. PCR screening of genomic DNA samples of T1 progeny of Triticum aestivum cv CPAN1676 line CP1 using primers specific to bar gene. The plasmid pDM302 and DNA isolated from an untransformed plant were used as positive and negative controls, respectively.

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