Figure 2
Genetic transformation of T. aestivum and T. durum by particle bombardment employing mature embryo-derived calli as the target tissue. The calli were bombarded with either pDM302/pAct1-F or pBY520 or pBI101::Act1. A. Mature embryo-derived callus of T. durum showing histochemical localization of the gus gene activity two days after bombardment with pAct1-F/pDM302. B & C. Treated and control explants, respectively, on regeneration medium supplemented with 2.5 mg/l phosphinothricin. D & E. Putative transformants of T. aestivum and T. durum, respectively, growing on half-strength MS medium supplemented with 2.5 mg/l phosphinothricin. F. Phosphinothricin leaf paint assay; transformant (left), control (right). G & H. Putative transformants of T. aestivum and T. durum, respectively. I. A T0 transgenic plant of T. durum obtained after bombardment with pBY520. J. T1 progeny plants of T. durum bombarded with pBY520.