Skip to main content

Table 2 CODEHOP primers used for UGTs amplification. y = [C, T], n = [A, G, C, T], r = [A, G], w = [A, T], k = [G, T]. BLAST analysis of protein consensus sequences corresponding to each primer was performed as indicated in the legend of Table 1.

From: Conservation and diversity of gene families explored using the CODEHOP strategy in higher plants

Primer Sequence Protein BLASTp hits on arabidopsis UGT database
Sense    
   PUGTa TG.TGC.GAG.TCC.ACC.ATG.aar.may.tgy.yt LCESTMKNCL 85A
   PUGTb GCC.GAG.GAG.CTC.GGC.rtn.ccn.gar.gt AEELGVPEV 85A
   PUGTc CC.TTC.TGC.CGG.ACC.ACC.ray.ccn.gay.ga SFCRTTNPDD 85A
   PUGTd CT.ATG.CAG.TCC.ATC.TTC.ccn.ccn.rtn.ta AMQSIFPPVY 85A
   PUGTe G.CCG.GAC.GTG.GTG.GCn.ggn.gan.gar.g LCESTMKNCL 85A
Reverse    
   PUGTar GC.GGA.GGT.GGT.CCA.GAA.Cwr.nac.ytc.ngg PEVQFWTTS 85A
   PUGTbr GAA.GTT.GAC.CAT.GAC.GTC.Gtc.ngg.rty.ngt TNPDDVMVSF 85A
   PUGTcr CGG.GCC.GAC.GGT.Gta.nay.ngg.ngg PPVYSVGP 85A/71B/71C/72B
   PUGTdr CGG.CAC.CAT.GGC.Ctc.ntc.ncc.nkc AGEEAMVP 85A
   PUGTer CCA.CTC.GTC.GCA.GCA.GAA.Cyk.rca.rtt.nrt MNCKFCCDEW 85A