Figure 6
Southern blot analysis of gene-disruption library transformants. 1 μg of genomic DNA isolated from two representative Physcomitrella transformants (BC344 and BC3238) was digested to completion with PvuII, which has a recognition site within the nptII coding sequence, or one of seven other restriction enzymes that do not cut within nptII and only rarely in Physcomitrella cDNAs. Fragments carrying nptII -sequences were detected by a DIG-labelled probe after electrophoresis and transfer to a nylon membrane.