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Figure 6 | BMC Plant Biology

Figure 6

From: High frequency of phenotypic deviations in Physcomitrella patens plants transformed with a gene-disruption library

Figure 6

Southern blot analysis of gene-disruption library transformants. 1 μg of genomic DNA isolated from two representative Physcomitrella transformants (BC344 and BC3238) was digested to completion with PvuII, which has a recognition site within the nptII coding sequence, or one of seven other restriction enzymes that do not cut within nptII and only rarely in Physcomitrella cDNAs. Fragments carrying nptII -sequences were detected by a DIG-labelled probe after electrophoresis and transfer to a nylon membrane.

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