Seedlings become resistant to the inhibitory effects of glucose on breakdown of seed storage lipid and synthesis of membrane lipid within three days of the start of imbibition. Seeds were sown on minimal media supplemented with 0.03 M glucose, 0.27 M glucose or 0.24 M sorbitol + 0.03 M glucose. After 3 days, some of the seedlings grown on 0.03 M glucose were transferred to media supplemented with 0.27 M glucose or 0.24 M sorbitol + 0.03 M glucose. Fatty acid levels were measured in seeds/seedlings harvested after an additional 7 days of growth. Note that this figure depicts the amount of 20:1 fatty acid metabolized by an average seedling, rather than the amount of 20:1 fatty acid remaining in an average seedling. See the "Materials and Methods" section for a description of the method used to estimate accumulation of membrane fatty acids. Results presented are means ± SD (n = 3, except n = 2 for seedlings transferred from 0.03 M glucose to 0.24 M sorbitol + 0.03 M glucose). Glc, glucose; Sorb, sorbitol. This experiment was repeated, with similar results.