Figure 7

Differential expression of subunit A transcripts in response to environmental stress conditions. Whole RNA was extracted from stress treated seedlings and control plants. Complementary DNA's were generated and transcripts amplified by PCR as described in materials and methods. All sixteen amplification products were fractionated on a single agarose gel, transferred to a solid support and hybridized with a digoxigenin labeled probe corresponding to the transcript-4 amplification product. Multiple time exposures were taken and the best was used for each panel, thus this figure does not represent equivalent time exposures for all four transcripts. These results are of a single experiment which has not been replicated. Lanes correspond to the following: N-normal, untreated control plants, S-sodium chloride treated plants, C-cold, E-etiolation.