Whole genome blot analysis of vacuolar type H+-ATPase subunit A gene in A. thaliana. Each lane represents 5 μg genomic DNA digested with the indicated restriction enzyme. Restriction enzymes used were; H, HindIII, B, BglII, D, DraI, K, KpnI, P, PvuII, E, EcoRI, Ps, PstI, Ev, EcoRv, Bc, BclI, and Ha, HaeIII. Hybridization was to a single band in each lane except for HaeIII due to the presence of a recognition site in the region corresponding to the probe. Positive hybridization control was 20 pg of unlabeled probe DNA generated via PCR. HindIII digested lambda DNA (2 μg) was used as negative control. Numbers to the left of the blot indicate the approximate size of DNA fragments (in kilobases) as determined by HindIII digestion of Lambda DNA.