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Table 2 The PTGS-mediated endogene cosuppression phenotype of the E82 locus is reduced by trans-silenced L91' as well as by naïve L91.

From: Epigenetic history of an Arabidopsis trans-silencer locus and a test for relay of trans-silencing activity

Grandparents F2 family endogene silencing phenotype
  Endogene [%] cop Remarks1)
  cop wt Observed Expected Excess of
L91' × E82 23 138 14% 75% wt plants
E82' × L91 30 42 42% 27% cop plants
L91 × E82 38 73 38% 87% wt plants
L91' × E82' 1 17 6% 0% one cop plant
  1. 1) Data were Chi-square tested under the null-hypothesis that L91' and E82' do not trans-silence their target loci, i.e., an additive interaction between the partner loci. Thus, in the case of L91 × E82, the only genotype escaping cosuppression should be L91 /-; E82 (-/-), which occurs at a frequency of 2/15 (13%). Note that entirely non-transgenic plants are not considered because of counterselection. Imprinted loci are not expected to cause endogene silencing (see Fig. 5). Thus, 12/15 of L91'/E82 family members, 4/15 of E82'/L91 family members and none of L91'/E82' family members are expected to be cop1-like. An excess of wild-type plants is inconsistent with an additive interaction and thus suggests trans-silencing activity by the L91' or L91 locus.