Figure 3

Structure of the GFP-COP1 locus E82. (A) The arrangement shown at the top was suggested by Southern blot analysis of NdeI (N) digested genomic DNA with a right border probe as indicated. In addition, PCR assays using sequence specific primers resulted in the predicted products as indicated by solid lines. Sizes of predicted PCR products are given in basepairs. Digits above the PCR products refer to the lane number in panel (B) showing the relevant fragment. The E82 locus lies on Chromosome 1 at basepair 42,325 of BAC T6A9 (Genbank: AC064879). (B) Ethidium bromide stained gel images showing predicted PCR products generated from the border sequences (lanes 1 to 3, fragment sizes indicated at left) and the internal RB/LB junction (lane 4, see marker at right).