Alternative splicing analysis. (A) Number of isoforms per gene distribution. (B) Donor and acceptor splicing site distributions. (C) Schematic representation of the most frequent splicing event identified in the v2 prediction: intron retention (IR), alternative 3' splicing site (Alt 3' ss), alternative 5' splicing site (Alt 5'), exon skipping (ES). The number of events is reported between brackets. (D) Pie chart showing the percentage distribution of alternative splicing events. (E) Intron size box plot distribution: all introns (ALL), constitutive introns (IC), alternatively spliced introns (ASI), introns that underwent intron retention events (IR), alternatively spliced introns without IR (AS-IR).