Figure 2

Phenotypes of cfm4 mutant plants and complementation lines. (A, B) Growth of the wild-type plants (WT), cfm4 mutants, and complementation line (Com1) at 20 and 33 days after germination (DAG). Scale bar = 1 cm. (C) The size and number of leaves at emergence of floral buds on 26 DAG. Scale bar = 1 cm. (D) Root growth (cm) of the plants measured on 20 DAG. Scale bar = 1 cm. (E) The size of cells in the roots of the plants measured on 26 DAG. Scale bar = 50 μm. (F) The amount of ABA was measured in 26-day-old plants, and relative amount to wild-type value is shown. (G) Transcript levels of ABA biosynthesis-related genes, ABA1, ABA2, ABA3, and NCED3, were measured in 26-day-old plants by real-time RT-PCR, and relative expression levels to wild-type value are shown. The values are mean ± SE obtained from five independent experiments, and asterisk above the number indicates statistically different values between WT and mutant plants (P ≤ 0.05).