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Figure 2 | BMC Plant Biology

Figure 2

From: Synthetic versions of firefly luciferase and Renilla luciferase reporter genes that resist transgene silencing in sugarcane

Figure 2

Dual Luciferase assays in sugarcane suspension cells. A. Equimolar amounts of pUbi-luc or pUbi-luc* were co-bombarded with equimolar amounts of pUbi-Renluc*. After using pUbi-Renluc* activity to normalise expression between bombardments, the results show on average 60-fold higher luciferase activity generated from the synthetic luc* transgene relative to the native firefly luc transgene. B. Equimolar amounts of pUbi-Renluc or pUbi-Renluc* were co-bombarded with equimolar amounts of pUbi-luc*. After using pUbi-luc* activity to normalise expression between bombardments, the results show on average 15-fold higher luciferase activity generated from the Renluc* transgene relative to the native Renluc transgene. Error bars represent the standard error of the mean from four co-bombardments of sugarcane suspension cells.

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