Localization and FRET analysis for AtGALT29A and AtGALT31A. A-B: Confocal images of AtGALT31A-mCer3 and AtGALT29A-YFP co-expressed in N . benthamiana leaves. C: The overlay image of (A) and (B). AtGALT31A-mCer3 and AtGALT29A-YFP are co-localized in high frequency. D-G: Distribution histogram for pixel by pixel analysis of FRET . FRET efficiency is expressed as FRET=, for example, FRET = 0.19 in (D) means that FRET efficiency is 19%; SEM, standard error of means; cell = number of cells analyzed. Scale bar = 5 μm.