Figure 7
Western blot analysis of lipoxygenase expression in Pinot Noir berry skin extracts. A: Analysis of plastid lipoxygenases expression during berry development using a commercial antibody against Arabidopsis LOX2 and 10 μg of total protein extracts per lane. B: Analysis of plastid lipoxygenase expression in chloroplast-enriched samples obtained from fresh berry skins collected at 9 wpf. Total chloroplast protein extract (Chl) was fractionated into membrane (P) and soluble (S) fractions by centrifugation. Membrane pellets were treated with 1 M NaCl or 0.05% Triton X-100, incubated for 10 min on ice and centrifuged again to separate the membrane (P) and the soluble (S) fractions. Pellets were resuspended in a volume identical to the corresponding soluble fractions and loaded in equal amounts for separation by SDS-PAGE and detection by western blot. MW markers: molecular weight markers (kDa).