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Figure 5 | BMC Plant Biology

Figure 5

From: Molecular characterization of mutations in white-flowered torenia plants

Figure 5

Transient expression assay for activation ability of F3H promoters in Arabidopsis suspension cells. Suspension-cultured cells of Arabidopsis thaliana (T87 line) were used for this analysis. GtMYB3 and GtbHLH1 genes that can activate late-stage anthocyanin biosynthetic genes were co-introduced with various F3H promoters via PEG-mediated transformation. After 24 h culture, firefly luciferase (FLUC) activity was measured with a luminometer. A Renilla luciferase (RLUC)-driven Cauliflower mosaic virus (CaMV) 35S promoter was used for internal standardization of PEG infection. Relative activities (LUC/RLUC) are shown. Asterisks (**) indicate significant differences between TfF3H pro (1 kbp) and other constructs (P < 0.01, t-test).

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