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Figure 8 | BMC Plant Biology

Figure 8

From: Identification and functional characterization of a flax UDP-glycosyltransferase glucosylating secoisolariciresinol (SECO) into secoisolariciresinol monoglucoside (SMG) and diglucoside (SDG)

Figure 8

UPLC chromatograms identifying the reaction products of UGT74S1 with SECO as SDG (peak 1) and SMG (peak 2). A, negative control including reaction buffer, SECO, UDP-glucose, and no enzyme; B, enzyme reaction including reaction buffer, SECO, UDP-glucose, and 50 μg of crude UGT74S1 enzyme. Peaks 1, 2, and 3 refer to the SDG, SMG and SECO peaks, respectively; C, enzyme reaction spiked with SDG standard prior to UPLC analysis D, SDG standard; E, SMG standard; F, SECO standard. The structures for SDG, SMG, and SECO are shown on the right.

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