Figure 8From: Identification and functional characterization of a flax UDP-glycosyltransferase glucosylating secoisolariciresinol (SECO) into secoisolariciresinol monoglucoside (SMG) and diglucoside (SDG)UPLC chromatograms identifying the reaction products of UGT74S1 with SECO as SDG (peak 1) and SMG (peak 2). A, negative control including reaction buffer, SECO, UDP-glucose, and no enzyme; B, enzyme reaction including reaction buffer, SECO, UDP-glucose, and 50 μg of crude UGT74S1 enzyme. Peaks 1, 2, and 3 refer to the SDG, SMG and SECO peaks, respectively; C, enzyme reaction spiked with SDG standard prior to UPLC analysis D, SDG standard; E, SMG standard; F, SECO standard. The structures for SDG, SMG, and SECO are shown on the right.Back to article page