Figure 3

Distributions of enzymes in total protein extract and three protein fractions. Immunoblotting of total protein extract (Total), soluble protein fraction (SP), loosely-bound protein fraction (LBP), and tightly-bound protein fraction (TBP) from three developing rice endosperms at 12–15 days after flowering (DAF). Samples are from double mutant, parental mutant, and wild-type lines. Immunoblotting analyses were conducted using antisera raised against rice SSI, GBSSI, BEI, and BEIIb. GBSSI from mature endosperms (mature GBSSI) were also analyzed. Amounts of protein loaded onto the gels were as follows: 1/25 of extracts in SP, 1/15 of extracts in LBP and 1/4 of extracts in TBP. Amount of protein bands in ‘Total’ was standardized against density of total protein bands stained with Coomassie Brilliant Blue (data not shown). Kin, Kinmaze; Nip, Nipponbare; T65, Taichung 65; WT, wild type.