Figure 2

Enzyme activities on zymograms. Native-PAGE/activity staining of starch synthases (SSs; Panel A), branching enzymes (BEs; Panel B), and debranching enzymes (DBEs; Panel C) in developing endosperm of double mutant, parental mutant, and wild-type lines. Arrowheads indicate SSI, SSIIIa, BEI, BEIIa, BEIIb, ISA (isoamylase), PUL (pullulanase), and PHO (phosphorylase) activity bands. Soluble protein extracts were prepared from developing endosperm. Amount of soluble protein is per mg fresh weight. Volumes of crude extract applied to the native gels in section ‘a’ are 2-fold and 4-fold greater than those applied in sections ‘b’ and ‘c’, respectively. Kin, Kinmaze; Nip, Nipponbare; T65, Taichung 65; WT, wild type.