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Table 1 Canola CBL and CIPK genes identified and their characteristics

From: Identification and characterization of CBL and CIPK gene families in canola (Brassica napus L.)

gene

GenBank Acc No.

EST count

Arabidopsis ortholog/AGI No.

Rice ortholog/locus

No. Amino acids

Protein M.W.(kDa)

pI

No. of EF-hands

Palmitoylation sites

Myristoylation sites

BnaCBL1

JQ708046

6

AtCBL1/At4g17615

OsCBL1/LOC_Os10g41510

213

24.6

4.57

4

yes

yes

BnaCBL2

JQ708048

11

AtCBL2/At5g55990

n.d.

226

25.9

4.79

4

yes

n.d.

BnaCBL3

JQ708049

25

AtCBL3/At4g26570

OsCBL3/LOC_Os03g42840

226

25.8

4.67

4

yes

n.d.

BnaCBL4

JQ708050

3

AtCBL4/At5g24270

OsCBL7/LOC_Os02g18880

221

25.4

4.68

4

yes

yes

BnaCBL9

JQ708051

18

AtCBL9/At5g47100

n.d.

213

24.4

4.5

4

yes

yes

BnaCBL10

JQ708047

16

AtCBL10/At4g33000

OsCBL10/LOC_Os01g51420

249

28.8

4.87

4

yes

n.d.

BnaCIPK1

JQ708052

28

AtCIPK1/At3g17510

OsCIPK21/LOC_Os07g44290

444

50

6.3

0

yes

n.d.

BnaCIPK3

JQ708061

13

AtCIPK3/At2g26980

OsCIPK32/LOC_Os12g03810

440

50.3

6.69

0

n.d.

n.d.

BnaCIPK5

JQ708062

11

AtCIPK5/At5g10930

OsCIPK16/LOC_Os09g25090

431

49.4

6.88

0

yes

n.d.

BnaCIPK6

JQ708063

199

AtCIPK6/At4g30960

OsCIPK5/LOC_Os01g10890

437

49

8.79

0

yes

n.d.

BnaCIPK7

JQ708064

13

AtCIPK7/At3g23000

OsCIPK4/LOC_Os12g41090

414

46.5

9.33

0

yes

n.d.

BnaCIPK8

JQ708065

26

AtCIPK8/At4g24400

OsCIPK8/LOC_Os01g35184

451

51

8.32

0

n.d.

n.d.

BnaCIPK9

JQ708066

28

AtCIPK9/At1g01140

OsCIPK9/LOC_Os03g03510

447

50.4

7.93

0

yes

n.d.

BnaCIPK10

JQ708053

14

AtCIPK10/At5g58380

OsCIPK2/LOC_Os07g48100

463

52.9

8.13

0

yes

n.d.

BnaCIPK11

JQ708054

30

AtCIPK11/At2g30360

n.d.

443

49.8

8.26

0

yes

n.d.

BnaCIPK12

JQ708055

35

AtCIPK12/At4g18700

OsCIPK12/LOC_Os01g55450

491

55

7.81

0

yes

n.d.

BnaCIPK14

KC414027

9

AtCIPK14/At5g01820

OsCIPK19/LOC_Os05g43840

431

48.6

9.06

0

yes

n.d.

BnaCIPK15

JQ708056

9

AtCIPK15/At5g01810

n.d.

423

48

8.61

0

n.d.

n.d.

BnaCIPK17

JQ708057

6

AtCIPK17/At1g48260

n.d.

427

47.7

7.68

0

yes

n.d.

BnaCIPK23

JQ708058

15

AtCIPK23/At1g30270

OsCIPK23/LOC_Os07g05620

482

53.4

9.25

0

yes

n.d.

BnaCIPK24

JQ708059

11

AtCIPK24/At5g35410

OsCIPK24/LOC_Os06g40370

453

51.6

9.2

0

n.d.

n.d.

BnaCIPK25

KC414028

18

AtCIPK25/At5g25110

n.d.

455

51.6

8.54

0

yes

n.d.

BnaCIPK26

JQ708060

18

AtCIPK26/At5g21326

OsCIPK32/LOC_Os12g03810

441

50

8.11

0

n.d.

n.d.

  1. The calcineurin B-like proteins (CBLs) and CBL-interacting protein kinases (CIPKs) genes of canola (Brassica napus L.) were identified through mining the expression sequence tags (ESTs), with cDNA sequences cloned through RACE-PCR and deposited in the GenBank. The nomenclature of BnaCBL and CIPK followed that of Arabidospis through a comparison in InParanoid7 (http://inparanoid.sbc.su.se/cgi-bin/index.cgi). The molecular weight (M.W.) and isoelectric point (pI) of the encoded proteins were predicted by DNAMAN software. The number of EF-hands was predicted by PROSITE scan. The palmitoylation sites were predicted by CSS-Palm 3.0 and myristoylation sites by Myristoylator. n.d., not detected.