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Table 1 Canola CBL and CIPK genes identified and their characteristics

From: Identification and characterization of CBL and CIPK gene families in canola (Brassica napus L.)

gene GenBank Acc No. EST count Arabidopsis ortholog/AGI No. Rice ortholog/locus No. Amino acids Protein M.W.(kDa) pI No. of EF-hands Palmitoylation sites Myristoylation sites
BnaCBL1 JQ708046 6 AtCBL1/At4g17615 OsCBL1/LOC_Os10g41510 213 24.6 4.57 4 yes yes
BnaCBL2 JQ708048 11 AtCBL2/At5g55990 n.d. 226 25.9 4.79 4 yes n.d.
BnaCBL3 JQ708049 25 AtCBL3/At4g26570 OsCBL3/LOC_Os03g42840 226 25.8 4.67 4 yes n.d.
BnaCBL4 JQ708050 3 AtCBL4/At5g24270 OsCBL7/LOC_Os02g18880 221 25.4 4.68 4 yes yes
BnaCBL9 JQ708051 18 AtCBL9/At5g47100 n.d. 213 24.4 4.5 4 yes yes
BnaCBL10 JQ708047 16 AtCBL10/At4g33000 OsCBL10/LOC_Os01g51420 249 28.8 4.87 4 yes n.d.
BnaCIPK1 JQ708052 28 AtCIPK1/At3g17510 OsCIPK21/LOC_Os07g44290 444 50 6.3 0 yes n.d.
BnaCIPK3 JQ708061 13 AtCIPK3/At2g26980 OsCIPK32/LOC_Os12g03810 440 50.3 6.69 0 n.d. n.d.
BnaCIPK5 JQ708062 11 AtCIPK5/At5g10930 OsCIPK16/LOC_Os09g25090 431 49.4 6.88 0 yes n.d.
BnaCIPK6 JQ708063 199 AtCIPK6/At4g30960 OsCIPK5/LOC_Os01g10890 437 49 8.79 0 yes n.d.
BnaCIPK7 JQ708064 13 AtCIPK7/At3g23000 OsCIPK4/LOC_Os12g41090 414 46.5 9.33 0 yes n.d.
BnaCIPK8 JQ708065 26 AtCIPK8/At4g24400 OsCIPK8/LOC_Os01g35184 451 51 8.32 0 n.d. n.d.
BnaCIPK9 JQ708066 28 AtCIPK9/At1g01140 OsCIPK9/LOC_Os03g03510 447 50.4 7.93 0 yes n.d.
BnaCIPK10 JQ708053 14 AtCIPK10/At5g58380 OsCIPK2/LOC_Os07g48100 463 52.9 8.13 0 yes n.d.
BnaCIPK11 JQ708054 30 AtCIPK11/At2g30360 n.d. 443 49.8 8.26 0 yes n.d.
BnaCIPK12 JQ708055 35 AtCIPK12/At4g18700 OsCIPK12/LOC_Os01g55450 491 55 7.81 0 yes n.d.
BnaCIPK14 KC414027 9 AtCIPK14/At5g01820 OsCIPK19/LOC_Os05g43840 431 48.6 9.06 0 yes n.d.
BnaCIPK15 JQ708056 9 AtCIPK15/At5g01810 n.d. 423 48 8.61 0 n.d. n.d.
BnaCIPK17 JQ708057 6 AtCIPK17/At1g48260 n.d. 427 47.7 7.68 0 yes n.d.
BnaCIPK23 JQ708058 15 AtCIPK23/At1g30270 OsCIPK23/LOC_Os07g05620 482 53.4 9.25 0 yes n.d.
BnaCIPK24 JQ708059 11 AtCIPK24/At5g35410 OsCIPK24/LOC_Os06g40370 453 51.6 9.2 0 n.d. n.d.
BnaCIPK25 KC414028 18 AtCIPK25/At5g25110 n.d. 455 51.6 8.54 0 yes n.d.
BnaCIPK26 JQ708060 18 AtCIPK26/At5g21326 OsCIPK32/LOC_Os12g03810 441 50 8.11 0 n.d. n.d.
  1. The calcineurin B-like proteins (CBLs) and CBL-interacting protein kinases (CIPKs) genes of canola (Brassica napus L.) were identified through mining the expression sequence tags (ESTs), with cDNA sequences cloned through RACE-PCR and deposited in the GenBank. The nomenclature of BnaCBL and CIPK followed that of Arabidospis through a comparison in InParanoid7 (http://inparanoid.sbc.su.se/cgi-bin/index.cgi). The molecular weight (M.W.) and isoelectric point (pI) of the encoded proteins were predicted by DNAMAN software. The number of EF-hands was predicted by PROSITE scan. The palmitoylation sites were predicted by CSS-Palm 3.0 and myristoylation sites by Myristoylator. n.d., not detected.