Functional complementation of Arabidopsis sos2-1 mutant by canola CIPK24 gene. (A) 4 DPS (days post-stratification) seedlings of wild-type (wt), sos2-1 mutants and complementary lines com-3, com-6 and com-9 were transferred to ½ x MS medium containing 0, 50 and 100 mM NaCl, respectively. The pictures were taken after 7 days of treatment. (B) qRT-PCR assay of BnaCIPK24 transgene expression levels in three independent transgenic lines in the wild-type Arabidopsis. Data is the mean (com/wt, linear scale) of three biological replicates ± S.E. Asterisks denote significant differences of BnaCIPK24 transcript abundance between transgenic lines and wt seedlings by Student t-test (p ≤ 0.05). The transcript levels of AtCIPK24 (SOS2) in the transgenic lines compared to that in wt was also calculated. (C) Seedling root elongation was measured at day 7 after the transfer. Error bars represent S.E. (n = 24). Asterisks denote significant differences between wt or transgenic and sos2 mutant plants by Student t-test (p ≤ 0.05).