Figure 6

Yeast two-hybrid analysis of interactions between CBLs and CIPK3 protein as well as its deletion versions. (A) Schematic diagram of the domain structure of BnaCIPK3. It contains an N-terminal kinase domain and a C-terminal regulatory domain, separated by a short junction domain. In the regulatory domain, the position of the short NAF/FISL motif is indicated. FL represents the full-length BnaCIPK3 protein, and D2 through D6 represent the five deletion constructs cloned in the pGADT7 vector. (B-F) represent the interaction assay between BnaCBL1, -2, -4, -9 or -10 and various fragments of BnaCIPK3, respectively. In each panel, the yeast cells harboring the indicated plasmid combinations were grown on either the nonselective (SD-LT) or selective (SD-LTHA) media, followed by β-galactosidase assay (X-Gal staining). Decreasing cell densities in the dilution series are illustrated by narrowing triangles. EV is the pGADT7 vector.