Yeast two-hybrid analysis of interactions between canola CBL and CIPK proteins. The yeast cells of strain AH109 harboring the indicated plasmid combinations were grown on either the nonselective (SD-LT) or selective (SD-LTHA) media, followed by β-galactosidase assay (X-Gal staining). Decreasing cell densities in the dilution series are illustrated by narrowing triangles. The A-F panels represent the titration assay results of interactions between BnaCBL1, -2, -3, -4, -9, -10 and 17 BnaCIPKs, respectively. The last row in each panel is the control test using pGADT7 empty vector (EV).