Figure 3
From: Identification and characterization of CBL and CIPK gene families in canola (Brassica napus L.)
Subcellular localization of selected BnaCBL- and BnaCIPK-GFP fusion proteins in N. benthamiana leaf cells. (A) Co-localization analysis of BnaCBL1-GFP (green) and AtCBL1n-mCherry (red). (B) Co-localization analysis of BnaCBL9-GFP (green) and AtCBL1n-mCherry (red). (C) Co-localization analysis of BnaCBL10-GFP (green) and AtTPC1-mCherry (red). (D)-(E) Co-localization analysis of BnaCIPK3-GFP and BnaCIPK10-GFP with AtCBL1n-mCherry fusion protein, respectively. A white arrow marks the region and direction in which the distribution of fluorescence intensities was scanned. In each case, the extreme left panel is GFP fluorescence, the middle mCherry field and the right represents an overlay of the two images. Bar = 50 μm.