Domain analysis and phylogenetic relationship of canola CBL proteins with CBLs from other species. (A) Multiple sequence alignment was performed using the ClustalX1.83 and illustrated by BOXSHADE (http://www.ch.embnet.org/software/BOX_form.html). Identical amino acids are shaded in black, and similar amino acids are shaded in gray. The four EF-hand motifs are indicated by overbars. The myristoylation site is in the rectangle. (B) Phylogenetic relationship of canola CBL proteins with CBLs from other species. Protein sequences were aligned using ClustalX (v1.83) and a maximum parsimony (MP) bootstrap consensus tree was drawn using MEGA5.1. The CBLs can be clustered into four major groups (I-IV). The percentage of replicate trees is shown on the branches and it is calculated in the bootstrap test (1000 replicates) for the associated taxa being clustered together. At, Arabidopsis thaliana; Bna, Brassica napus; Gh, Gossypium hirsutum; Ol, Ostreococcus lucimarinus; Os, Oryza sativa; ; Ot, Ostreococcus tauri; Ps, Psium sativum; Pv, Phaseolus vulgaris; Sb, Sorghum bicolor; Zm, Zea maize.