Figure 7

Real-time qRT-PCR analysis of ABA-responsive genes in wild type and APUM5 transgenic plants under drought stress. (A) Expression of the ABA-regulated genes in wild-type and APUM5 transgenic plants under drought stress. mRNA levels were determined by qRT-PCR using total RNAs isolated from control and 6 h drought-stressed detached rosette leaves of wild-type and APUM5 transgenic plants. Data are mean values of three independent experiments (Student’s t-test; *P < 0.01). (B) Sequence analysis of the putative APUM5 binding sites in the 3′ UTRs of abiotic stress-related genes. The putative binding sequence including ‘UGUA’ is highlighted. hbNRE2 was used as a conserved Pumilio RNA binding motif. The ABA-responsive genes down-regulated in the 35S-APUM5 transgenic plants under drought stress were analyzed at the 3′ UTR sequence data base of The Arabidopsis Information Resource (TAIR) and UTRdb (http://utrdb.ba.itb.cnr.it/). The motif was used to search for Drosophila hbNRE2-related sequences and reconstructed using sequence alignment (CLUSTALW) and WebLogo software.