Figure 5

GmMYB73 enhances lipid contents in seeds and leaves of transgenic Lotus plants, and in transgenic hairy roots of soybean plants. (a) GmMYB73 expression in leaves of transgenic Lotus plants. Two lines OE-7 and OE-21 were used. Tubulin gene was amplified as a control. (b) Contents of total lipids in transgenic seeds compared to WT. Error bars indicate SD (n = 4) and the values are in dry weight. Asterisks indicate significant difference compared to WT (**P < 0.01, *P < 0.05). (c) Contents of total fatty acids in transgenic seeds. Error bars indicate SD (n = 4) and others are as in (b). (d) Contents of each fatty acid composition in transgenic seeds. Others are as in (b). (e) Contents of total fatty acids in leaves of transgenic Lotus plants. The values are in fresh weight. Others are as in (b). (f) Contents of each fatty acid in leaves of transgenic Lotus plants. The values are in fresh weight. Others are as in (b). (g) GmMYB73 expression in transgenic hairy roots of soybean plants. K599: control roots. GmMYB73: GmMYB73-transgenic hairy roots. Error bars indicate SD (n = 4). Asterisk indicates significant difference compared to control K599 (*P < 0.05). (h) Total fatty acid levels in GmMYB73-transgenic hairy roots. K599: control roots. GmMYB73: GmMYB73-transgenic hairy roots. GmDof4: GmDof4-transgenic hairy roots as a positive control for lipid accumulation. Error bars indicate SD (n = 4). The values are in fresh weight. Asterisks indicate significant difference compared to control K599 (**P < 0.01, *P < 0.05). (i) Levels of each fatty acid composition in GmMYB73-transgenic hairy roots. The values are in fresh weight. Other indications are as in (h).