Figure 2

GmMYB73 interacts with GL3 and EGL3, and inhibits GL2 expression. (a) Interaction of GmMYB73 with GL3 and EGL3 in yeast two-hybrid assay. Yeast transformants were grown on control SD/–Leu/–Trp (left column), or selection medium SD/–Ade/–His/–Leu/–Trp with X-a-Gal and Aureobasidin A (right). Growth of cells and blue color on selection medium indicate positive interactions. Other combinations were used as negative controls. (b) BiFC was used to detect the interaction between GmMYB73 and GL3 or EGL3 in Arabidopsis protoplasts. Yellow fluorescence in YFP indicates positive interactions. (c) GL2 promoter activity was inhibited by GmMYB73 in aerial parts but partially suppressed in roots. Left: GUS staining in whole seedlings; right: GUS staining in roots. (d) cGmMYB73 suppressed GL2 promoter activity and trichome formation on sepals of floral buds (left), leaves (middle) and stems (right). (e) GL2 promoter activity was inhibited by GmMYB73 as revealed by GUS activity in leaves, roots and hypocotyls of transgenic plants. Asterisks ‘**’ indicate a significant difference from Col-0 levels (P < 0.01). (f) GmMYB73 inhibited GL2 promoter activity during silique development as revealed from GUS staining. (g) GmMYB73 inhibited GL2 promoter activity as revealed from relative GUS activity. GUS activity at stage 1 of GL2p::GUS/GmMBY73 was set to 1 and all the other values were compared with it. The five stages corresponded to the silique phenotypes in (f) respectively.