Figure 8

Early changes in the transcriptome of Arabidopsis roots under Pi starvation. A) Workflow for experiments. Arabidopsis seedlings were grown hydroponically for 12 days under sufficient Pi level (125 μM Pi) and then transferred to Pi-depleted liquid media for short times. Roots were harvested, RNA isolated and transcriptome analyzed using an oligonucleotide microarray platform. B) Edwards-Venn diagrams showing common or distinct regulated genes over the sampled time points. C) Clustering of differentially expressed genes. Clustering was performed using the Smooth correlation and average linkage clustering in GeneSpring GX 7.3.1 software (Agilent Technologies). Orange indicates up-regulated, green indicates down-regulated and white unchanged values, as shown on the color scale at the right side of the figure.