Figure 5

Inhibitory effect of phosphite on primary root length and root hair density under phosphate deficiency. The effect of phosphite (Phi) treatments on primary root length (A) and root hair density (B) of Arabidopsis thaliana ecotype Columbia (Col-0) grown under different phosphate (+Pi and -Pi) regimes. (A) Primary root length measured 7 days after transferring the seedlings to different Pi (+Pi; 1.25 mM and –Pi; 0 mM) and Phi media. Data are the means of 10 roots assessed per treatment with standard errors and bars with the same letter are not significantly different according to Tukey HSD test. (B) Root hair density (number) 7 days after transferring the seedlings to different Pi (+Pi and –Pi) and Phi medium. Root hair density was determined as the number of hairs in a 5 mm root segment, 2.5 mm from the root tip, and each bar represents the mean of six plants with standard error bars and bars with the same letter are not significantly (P > 0.05) different according to Tukey HSD test. (C) Shows the effect of different Phi concentration on root hair formation in seedlings grown in + Pi or –Pi media.