The rtm1 eifiso4e double mutant is resistant to TEV HAT and TEV CAA10 does not infect Arabidopsis thaliana L er or Col accessions. One-month-old Arabidopsis were manually inoculated with TEV HAT or CAA10 strains and assayed at 24 dpi. Wild type accessions Columbia (eIFiso4E/eIFiso4E; RTM1/RTM1) and Landsberg erecta (eIFiso4E/eIFiso4E; rtm1/rtm1) were compared to the double mutant eifiso4e/eifiso4e; rtm1/rtm1. Nicotiana tabacum cv Xanthi non-inoculated (mock) or inoculated (Inoc) plants were included as controls. A, Plants were assayed for viral coat protein accumulation by ELISA at 24 dpi. Mean values for 6 independent plants per genotype are shown and error bars represent standard errors. The horizontal black line is the susceptibility threshold. B, RT-PCR expression of the TEV VPg gene in systemic leaf tissues. APT1 is amplified as a constitutive control in Arabidopsis plants.