Table 1 Characteristics of 74 polymorphic microsatellite loci developed in grasspea (FP = forward primer, RP = reverse primer, Ta = annealing temperature)
Primer | Repeat motif | Primer sequence (5′-3′) | Real product size(bp) | Ta/°C |
|---|---|---|---|---|
G1 | (A)10 | FP-AAGGAGCAGCAGCATTTGTT | 210-240 | 52 |
RP-TAATAATGGGGAGCCGATCA | ||||
G4 | (AAAG)5 | FP- CCTTTCGGAGCAATCAAGAC | 110-120 | 56 |
RP- TGCCTAAGCATTGGCTTTCT | ||||
G5 | (AAC)10 | FP- CACAACCAGTTGCATCAGTG | 200-220 | 56 |
RP- TGGCTCACATGATGGTTTGT | ||||
G6 | (AAC)12 | FP- TGGAGGACGAGCAACAATAA | 230-250 | 54 |
RP- TGTTGTTGATGGAAACAAATGA | ||||
G7 | (AAC)5 | FP- ACAGCAAGAAGCAGCAACAG | 230-245 | 56 |
RP- AGTTGGTTGTTGTGTCGTTGT | ||||
G9 | (AAC)6 | FP- CAACCAGAGCAACCACAAGA | 240-260 | 56 |
RP- GGTTGCAAGAGGTTGCAGAT | ||||
G13 | (AACCA)5 | FP-CAAACCAAACCAAACCAAACT | 180-195 | 52 |
RP- CGCGTTTTGGTTTTCGTACT | ||||
G15 | (AAG)6 | FP- TCAAGCCCAAAGTGAGATGA | 145-155 | 56 |
RP- TTTTGTGTTGCTTGCTGACC | ||||
G17 | (AAT)5 | FP- CAGGTCCGGCTTATCTCTCA | 180-200 | 56 |
RP- TTGGTTTCAACCCACTCCTC | ||||
G18 | (AC)10 | FP- ACACGACACACACGACAGTG | 130-140 | 52 |
RP- CTGCGTGTCTGTGCCTATTG | ||||
G27 | (AC)18 | FP- ATCTTACCGGGGATCCATTC | 190-210 | 56 |
RP- CTTCCCCATTCTCTGGTGTT | ||||
G33 | (AC)6 | FP- ACCAAAGGATGCAGGGTCTA | 230-270 | 54 |
RP- TAGTCGTGGTGTCGTGGTGT | ||||
G39 | (AC)6 | FP- CCAGACACACACGCAAACAT | 170-190 | 54 |
RP- GTGTGTGACGTTGCCGTTAG | ||||
G49 | (AC)7 | FP-ACGCACACACGGAAGAAAG | 160-180 | 56 |
RP-GTGTGCGCATGTGTGTATGA | ||||
G61 | (AC)8 | FP-CACACACCATTACGCACACA | 130-150 | 54 |
RP-TGGTGTCGTGGTCGTAGGTA | ||||
G64 | (AC)8 | FP-GCACATTCGCACGTATTCAC | 160-180 | 56 |
RP-CGTTTCTGAGTGCGTTGTGT | ||||
G67 | (AC)9 | FP-CACCCTCTTCACTGCCTAGC | 120-150 | 52 |
RP-TTGGGGGTTGTAGAAGGAAC | ||||
G68 | (AC)9 | FP-GCACACAAGGGCACACTG | 170-180 | 52 |
RP-TGCGTCGTGTGTATGTGTTG | ||||
G72 | (ACA)5 | FP-CAACGACAACAACGCAAAAC | 260-280 | 52 |
RP-TTCGCGGTTTGTCCATTTAG | ||||
G73 | (ACA)5 | FP-CCAACTCTCAGCCACGAACT | 200-220 | 54 |
RP-TTGCTCCACCTACGCTTCTT | ||||
G75 | (ACA)6 | FP-AACAACAGCAGCAACAACAAT | 200-215 | 54 |
RP-CGTGTTGTGTGTTCGTTCGTA | ||||
G76 | (ACA)6 | FP-CACAACCAACGCCAATACAG | 230-250 | 54 |
RP-CCGTAGTACCGCGCTTATTC | ||||
G77 | (AAC)11 | FP-ACAAGACAACATCACCGAGAC | 300-330 | 52 |
RP-TGTTGTTTGGTTGTTCGTGTA | ||||
G80 | (AAC)5 | FP-AAACACAACAGACGATTAAACACA | 185-200 | 52 |
RP-TCTTGCTATGTAGTGTTGTGTGATG | ||||
G81 | (ACG)5 | FP-CGCACACACTCACACACAAC | 180-200 | 52 |
RP-GGTCCTGTCGTCGTAGTCCT | ||||
G83 | (ACG)7 | FP-GGGCACACATTCTCACACAC | 190-200 | 54 |
RP-TGTCGTCGTGTCGTAGTCGT | ||||
G87 | (AG)15 | FP-CCCTTACCGAGTGCAGAAAA | 230-250 | 54 |
RP-CACCACGACTTGCTCACCTA | ||||
G101 | (CA)11c(CA)7 | FP-TGGCAGGTAACTGGTGAGTG | 180-190 | 52 |
RP-GGTGTTTCCCCACCTCTCTA | ||||
G102 | (CA)12 | FP-AAAGCACAGCACAACACGAC | 260-280 | 54 |
RP-AACAAGGACGACGGTAGGTG | ||||
G110 | (CA)6 | FP-CACAAACACGCACAAACACA | 150-170 | 52 |
RP-CGTCGGTATAACCGTGTCGT | ||||
G116 | (CA)6(CACACG)5 | FP-CACACAGGACAGCACTCACA | 150-180 | 52 |
RP-GTCGTCGGTGTGTCGTAGTC | ||||
G119 | (CA)6cgacacacncgcgcgcgcgacacac(ACG)8 | FP-CGTCTCTTCAAAGGGCCATA | 190-200 | 52 |
RP-CGACCGACCGACGTACTACT | ||||
G120 | (CA)6cgcacgcacgcacacagacacg(CA)7 | FP-GCGCACGCATACATACACA | 160-170 | 54 |
RP-TTGCCGTTGTCGTGTTAGTG | ||||
G123 | (CA)6gn(AC)6 | FP-CATAACAACACGCAGCATTACC | 130-140 | 52 |
RP-TTGCGTTGTTGTGTTGTGTTT | ||||
G128 | (CA)7 | FP-CCACACACCCACATGTTCA | 210-230 | 56 |
RP-TTGTGGTGGGTCTGAGAGTG | ||||
G131 | (CA)7aacacgttcg(CA)8 | FP-GCGCTCACACCAACATAAAG | 140-150 | 54 |
RP-TGTATGCGTGCGTATGTCTG | ||||
G133 | (CA)7cgcacat(AC)6 | FP-ACGCGTGCACACATTTTATC | 200-220 | 52 |
RP-TATGTGGGCGCGTGTAAGTA | ||||
G136 | (CA)7tacacacacat(AC)7aa(AC)6 | FP-ACGACGACCACCAGTACGA | 110-130 | 54 |
RP-ACGAGTGCGTGTGTGAGTGT | ||||
G142 | (CA)8cgcacaa(AC)10 | FP-CGTGCACGCACAGATACG | 160-180 | 52 |
RP-GTGTGTGTGTTCGTCGTTTG | ||||
G143 | (CA)8cggcgcgcg(AC)9 | FP-GACACACACAACCCGAACAC | 230-260 | 52 |
RP-TGAGCGAACGTACGTGGTAG | ||||
G145 | (CA)8tacgcacg(CA)10 | FP-ATACAAGCACGCATCCACAG | 100-120 | 52 |
RP-AGTTCGTGTCGTGTCGTGTC | ||||
G147 | (CA)9 | FP-CGTCACACACGTCACGTACA | 210-230 | 54 |
RP-CTACGAGACGCACGACGATA | ||||
G150 | (CA)9 g(AC)25 | FP-CACACACACCAAGCGTTACA | 140-160 | 54 |
RP-TCGTGTGTGTCGTGTGTGTAG | ||||
G151 | (CAA)10 | FP-CAACAACGACAACAAAATTGTAA | 175-185 | 52 |
RP-CTGCTGATGTTGTTGGTGCT | ||||
G154 | (CAA)5agaccacaacaccaccacc aacaacaacaataataaaacag(AAC)5 | FP-CTGGCGTAATAGCGAAGAGG | 240-260 | 56 |
RP-TGTGTTGCTTTGTGTTGTCGT | ||||
G157 | (CAA)6 | FP-ACATCCAATCCCCACCATAA | 210-230 | 56 |
RP-AATGCATGGTTGTTGCTTGA | ||||
G165 | (CGA)5 | FP-GAACGTACGACGACACGAACT | 270-290 | 54 |
RP-CGTGTGGTGTGTGTGTGTGT | ||||
G171 | (CT)9 | FP-CTTCACTGCATGCTTTCCAC | 200-230 | 52 |
RP-CTGGGGTGGTTTTTGTCAGT | ||||
G174 | (GA)19 | FP-CACAAGGGTCAAGGGAGAGA | 140-160 | 52 |
RP-GTTTACGTTACTTATTCGTTCGTTAG | ||||
G184 | (GT)15 | FP-GCGTGTGTGCGAATGTGT | 180-190 | 52 |
RP-CACGCACGCACACTAGACTAC | ||||
G185 | (GT)19 | FP-TGCGTGTGTCGCTCTATCAT | 130-135 | 56 |
RP-TACTGCGACAACCGAACGTA | ||||
G188 | (GT)6 | FP-GCGCGTTAGTGTGTGTTTGA | 140-150 | 52 |
RP-CACGCACGCACACTTACATA | ||||
G191 | (GT)6 | FP-TGTGCGTGGTGTTTGAGTG | 140-160 | 52 |
RP-CACATACGCACAGCCCATAC | ||||
G192 | (GT)6a(TG)7 | FP-TGCGTGATAAGGTGCTTGAG | 160-170 | 56 |
RP-ACACACACACGCACACACAC | ||||
G200 | (GT)7 | FP-GGATGGTGTGCTGTGTGTGT | 120-135 | 52 |
RP-AACACCAACTACCGGCAACT | ||||
G205 | (GT)7gcgtgtgcctgcgtctctgcgagtgcgtgc(GT)6 | FP-TGTCTGGTGTGTGTGGTGTG | 230-250 | 52 |
RP-CGACACGTACGCAACGAC | ||||
G206 | (GT)8 | FP-AAACTGGCCCTGCATTTTC | 190-210 | 52 |
RP-GGTCATGGCAATTTGAGACA | ||||
G209 | (GT)8 | FP-TTTGCACGTGTCCTGTGTTT | 240-260 | 52 |
RP-ACGACGACCACACACCACTA | ||||
G211 | (GT)9 | FP-ATGGCGTCGTATGTGTGTGT | 200-210 | 52 |
RP-GTTACGGCCGAATCAACAAC | ||||
G219 | (GTT)10 | FP-CCAGTTGTGCCGAACACAT | 130-160 | 52 |
RP-CCAACAGCAGATTGCCAGTA | ||||
G225 | (GTT)7 | FP-GGGCAGTGGACCAGTTAGAG | 250-270 | 52 |
RP-CCGAGGGAATAAACGACAAA | ||||
G228 | (T)10 | FP-CCTACGGACATGCCTGTTTT | 280-310 | 52 |
RP-GCGGTAGGGGAAAAACAACT | ||||
G233 | (TC)20 | FP-CGTTCGTCCTTCTCCTCCTA | 120-140 | 52 |
RP-AGACGACTACGGACGACGAC | ||||
G234 | (TC)7 | FP-GTTGGGTTTGGCATTGAACT | 190-210 | 52 |
RP-GAAGGGGCGAACAAATAAAA | ||||
G244 | (TG)6 | FP-CAATCCGAAAATCACCACCT | 230-250 | 52 |
RP-GCACTCACATGCACACAAAC | ||||
G245 | (TG)6 | FP-CGTTGGTTGTTAGTCGGTCA | 240-260 | 52 |
RP-GAACGAAACAACGACGACAA | ||||
G249 | (TG)6c(GT)7 | FP-TATGTGTGCAACGGCAACTT | 140-160 | 52 |
RP-GCACACCCACCACACAATAG | ||||
G254 | (TG)7 | FP-TGAGTGCGTACGTGTGTCTG | 100-120 | 52 |
RP-GCGCGTGTTCACACATAGAC | ||||
G262 | (TGGT)5 | FP-TGTGCGTGTGTGTGTTTTTG | 300-320 | 52 |
RP-ACCACAACCCCTACCCTACC | ||||
G268 | (TGT)5tattn(TTG)6 | FP-TTGTTTGTTGTTGTTGTGTCTTG | 290-305 | 52 |
RP-CTACAGTACAGACCCGCCACT | ||||
G269 | (TGT)6 | FP-ATGCTGTTGATGCGTCAGTT | 220-240 | 52 |
RP-TGCAGCAACAACAAATAAGACA | ||||
G273 | (TGT)7 | FP- TTTTTGGTATTGTTGTTGTCGT | 250-270 | 52 |
RP- CTGCAGCAATAACAGCATCAG | ||||
G284 | (TTG)6 | FP-TGTGTTGTGTTGTGCTGTATGTA | 160-170 | 52 |
RP-GCAGCAACATTAAAACGAACAG | ||||
G285 | (TTG)6 | FP-TTTGTGCGGTTGATGTTGTT | 190-200 | 52 |
RP-CTACGTCAGCCCGTCATACC |