Large-scale microsatellite development in grasspea ( Lathyrus sativus L.), an orphan legume of the arid areas

Tao Yang; Junye Jiang; Marina Burlyaeva; Jinguo Hu; Clarice J Coyne; Shiv Kumar; Robert Redden; Xuelian Sun; Fang Wang; Jianwu Chang; Xiaopeng Hao; Jianping Guan; Xuxiao Zong

doi:10.1186/1471-2229-14-65

Table 1 Characteristics of 74 polymorphic microsatellite loci developed in grasspea (FP = forward primer, RP = reverse primer, Ta = annealing temperature)

From: Large-scale microsatellite development in grasspea (Lathyrus sativus L.), an orphan legume of the arid areas

Primer	Repeat motif	Primer sequence (5′-3′)	Real product size(bp)	Ta/°C
G1	(A)10	FP-AAGGAGCAGCAGCATTTGTT	210-240	52
G1	(A)10	RP-TAATAATGGGGAGCCGATCA	210-240	52
G4	(AAAG)5	FP- CCTTTCGGAGCAATCAAGAC	110-120	56
G4	(AAAG)5	RP- TGCCTAAGCATTGGCTTTCT	110-120	56
G5	(AAC)10	FP- CACAACCAGTTGCATCAGTG	200-220	56
G5	(AAC)10	RP- TGGCTCACATGATGGTTTGT	200-220	56
G6	(AAC)12	FP- TGGAGGACGAGCAACAATAA	230-250	54
G6	(AAC)12	RP- TGTTGTTGATGGAAACAAATGA	230-250	54
G7	(AAC)5	FP- ACAGCAAGAAGCAGCAACAG	230-245	56
G7	(AAC)5	RP- AGTTGGTTGTTGTGTCGTTGT	230-245	56
G9	(AAC)6	FP- CAACCAGAGCAACCACAAGA	240-260	56
G9	(AAC)6	RP- GGTTGCAAGAGGTTGCAGAT	240-260	56
G13	(AACCA)5	FP-CAAACCAAACCAAACCAAACT	180-195	52
G13	(AACCA)5	RP- CGCGTTTTGGTTTTCGTACT	180-195	52
G15	(AAG)6	FP- TCAAGCCCAAAGTGAGATGA	145-155	56
G15	(AAG)6	RP- TTTTGTGTTGCTTGCTGACC	145-155	56
G17	(AAT)5	FP- CAGGTCCGGCTTATCTCTCA	180-200	56
G17	(AAT)5	RP- TTGGTTTCAACCCACTCCTC	180-200	56
G18	(AC)10	FP- ACACGACACACACGACAGTG	130-140	52
G18	(AC)10	RP- CTGCGTGTCTGTGCCTATTG	130-140	52
G27	(AC)18	FP- ATCTTACCGGGGATCCATTC	190-210	56
G27	(AC)18	RP- CTTCCCCATTCTCTGGTGTT	190-210	56
G33	(AC)6	FP- ACCAAAGGATGCAGGGTCTA	230-270	54
G33	(AC)6	RP- TAGTCGTGGTGTCGTGGTGT	230-270	54
G39	(AC)6	FP- CCAGACACACACGCAAACAT	170-190	54
G39	(AC)6	RP- GTGTGTGACGTTGCCGTTAG	170-190	54
G49	(AC)7	FP-ACGCACACACGGAAGAAAG	160-180	56
G49	(AC)7	RP-GTGTGCGCATGTGTGTATGA	160-180	56
G61	(AC)8	FP-CACACACCATTACGCACACA	130-150	54
G61	(AC)8	RP-TGGTGTCGTGGTCGTAGGTA	130-150	54
G64	(AC)8	FP-GCACATTCGCACGTATTCAC	160-180	56
G64	(AC)8	RP-CGTTTCTGAGTGCGTTGTGT	160-180	56
G67	(AC)9	FP-CACCCTCTTCACTGCCTAGC	120-150	52
G67	(AC)9	RP-TTGGGGGTTGTAGAAGGAAC	120-150	52
G68	(AC)9	FP-GCACACAAGGGCACACTG	170-180	52
G68	(AC)9	RP-TGCGTCGTGTGTATGTGTTG	170-180	52
G72	(ACA)5	FP-CAACGACAACAACGCAAAAC	260-280	52
G72	(ACA)5	RP-TTCGCGGTTTGTCCATTTAG	260-280	52
G73	(ACA)5	FP-CCAACTCTCAGCCACGAACT	200-220	54
G73	(ACA)5	RP-TTGCTCCACCTACGCTTCTT	200-220	54
G75	(ACA)6	FP-AACAACAGCAGCAACAACAAT	200-215	54
G75	(ACA)6	RP-CGTGTTGTGTGTTCGTTCGTA	200-215	54
G76	(ACA)6	FP-CACAACCAACGCCAATACAG	230-250	54
G76	(ACA)6	RP-CCGTAGTACCGCGCTTATTC	230-250	54
G77	(AAC)11	FP-ACAAGACAACATCACCGAGAC	300-330	52
G77	(AAC)11	RP-TGTTGTTTGGTTGTTCGTGTA	300-330	52
G80	(AAC)5	FP-AAACACAACAGACGATTAAACACA	185-200	52
G80	(AAC)5	RP-TCTTGCTATGTAGTGTTGTGTGATG	185-200	52
G81	(ACG)5	FP-CGCACACACTCACACACAAC	180-200	52
G81	(ACG)5	RP-GGTCCTGTCGTCGTAGTCCT	180-200	52
G83	(ACG)7	FP-GGGCACACATTCTCACACAC	190-200	54
G83	(ACG)7	RP-TGTCGTCGTGTCGTAGTCGT	190-200	54
G87	(AG)15	FP-CCCTTACCGAGTGCAGAAAA	230-250	54
G87	(AG)15	RP-CACCACGACTTGCTCACCTA	230-250	54
G101	(CA)11c(CA)7	FP-TGGCAGGTAACTGGTGAGTG	180-190	52
G101	(CA)11c(CA)7	RP-GGTGTTTCCCCACCTCTCTA	180-190	52
G102	(CA)12	FP-AAAGCACAGCACAACACGAC	260-280	54
G102	(CA)12	RP-AACAAGGACGACGGTAGGTG	260-280	54
G110	(CA)6	FP-CACAAACACGCACAAACACA	150-170	52
G110		RP-CGTCGGTATAACCGTGTCGT	150-170	52
G116	(CA)6(CACACG)5	FP-CACACAGGACAGCACTCACA	150-180	52
G116	(CA)6(CACACG)5	RP-GTCGTCGGTGTGTCGTAGTC	150-180	52
G119	(CA)6cgacacacncgcgcgcgcgacacac(ACG)8	FP-CGTCTCTTCAAAGGGCCATA	190-200	52
G119	(CA)6cgacacacncgcgcgcgcgacacac(ACG)8	RP-CGACCGACCGACGTACTACT	190-200	52
G120	(CA)6cgcacgcacgcacacagacacg(CA)7	FP-GCGCACGCATACATACACA	160-170	54
G120	(CA)6cgcacgcacgcacacagacacg(CA)7	RP-TTGCCGTTGTCGTGTTAGTG	160-170	54
G123	(CA)6gn(AC)6	FP-CATAACAACACGCAGCATTACC	130-140	52
G123	(CA)6gn(AC)6	RP-TTGCGTTGTTGTGTTGTGTTT	130-140	52
G128	(CA)7	FP-CCACACACCCACATGTTCA	210-230	56
G128	(CA)7	RP-TTGTGGTGGGTCTGAGAGTG	210-230	56
G131	(CA)7aacacgttcg(CA)8	FP-GCGCTCACACCAACATAAAG	140-150	54
G131	(CA)7aacacgttcg(CA)8	RP-TGTATGCGTGCGTATGTCTG	140-150	54
G133	(CA)7cgcacat(AC)6	FP-ACGCGTGCACACATTTTATC	200-220	52
G133	(CA)7cgcacat(AC)6	RP-TATGTGGGCGCGTGTAAGTA	200-220	52
G136	(CA)7tacacacacat(AC)7aa(AC)6	FP-ACGACGACCACCAGTACGA	110-130	54
G136	(CA)7tacacacacat(AC)7aa(AC)6	RP-ACGAGTGCGTGTGTGAGTGT	110-130	54
G142	(CA)8cgcacaa(AC)10	FP-CGTGCACGCACAGATACG	160-180	52
G142	(CA)8cgcacaa(AC)10	RP-GTGTGTGTGTTCGTCGTTTG	160-180	52
G143	(CA)8cggcgcgcg(AC)9	FP-GACACACACAACCCGAACAC	230-260	52
G143	(CA)8cggcgcgcg(AC)9	RP-TGAGCGAACGTACGTGGTAG	230-260	52
G145	(CA)8tacgcacg(CA)10	FP-ATACAAGCACGCATCCACAG	100-120	52
G145	(CA)8tacgcacg(CA)10	RP-AGTTCGTGTCGTGTCGTGTC	100-120	52
G147	(CA)9	FP-CGTCACACACGTCACGTACA	210-230	54
G147	(CA)9	RP-CTACGAGACGCACGACGATA	210-230	54
G150	(CA)9 g(AC)25	FP-CACACACACCAAGCGTTACA	140-160	54
G150	(CA)9 g(AC)25	RP-TCGTGTGTGTCGTGTGTGTAG	140-160	54
G151	(CAA)10	FP-CAACAACGACAACAAAATTGTAA	175-185	52
G151	(CAA)10	RP-CTGCTGATGTTGTTGGTGCT	175-185	52
G154	(CAA)5agaccacaacaccaccacc aacaacaacaataataaaacag(AAC)5	FP-CTGGCGTAATAGCGAAGAGG	240-260	56
G154	(CAA)5agaccacaacaccaccacc aacaacaacaataataaaacag(AAC)5	RP-TGTGTTGCTTTGTGTTGTCGT	240-260	56
G157	(CAA)6	FP-ACATCCAATCCCCACCATAA	210-230	56
G157	(CAA)6	RP-AATGCATGGTTGTTGCTTGA	210-230	56
G165	(CGA)5	FP-GAACGTACGACGACACGAACT	270-290	54
G165	(CGA)5	RP-CGTGTGGTGTGTGTGTGTGT	270-290	54
G171	(CT)9	FP-CTTCACTGCATGCTTTCCAC	200-230	52
G171	(CT)9	RP-CTGGGGTGGTTTTTGTCAGT	200-230	52
G174	(GA)19	FP-CACAAGGGTCAAGGGAGAGA	140-160	52
G174	(GA)19	RP-GTTTACGTTACTTATTCGTTCGTTAG	140-160	52
G184	(GT)15	FP-GCGTGTGTGCGAATGTGT	180-190	52
G184	(GT)15	RP-CACGCACGCACACTAGACTAC	180-190	52
G185	(GT)19	FP-TGCGTGTGTCGCTCTATCAT	130-135	56
G185	(GT)19	RP-TACTGCGACAACCGAACGTA	130-135	56
G188	(GT)6	FP-GCGCGTTAGTGTGTGTTTGA	140-150	52
G188	(GT)6	RP-CACGCACGCACACTTACATA	140-150	52
G191	(GT)6	FP-TGTGCGTGGTGTTTGAGTG	140-160	52
G191	(GT)6	RP-CACATACGCACAGCCCATAC	140-160	52
G192	(GT)6a(TG)7	FP-TGCGTGATAAGGTGCTTGAG	160-170	56
G192	(GT)6a(TG)7	RP-ACACACACACGCACACACAC	160-170	56
G200	(GT)7	FP-GGATGGTGTGCTGTGTGTGT	120-135	52
G200	(GT)7	RP-AACACCAACTACCGGCAACT	120-135	52
G205	(GT)7gcgtgtgcctgcgtctctgcgagtgcgtgc(GT)6	FP-TGTCTGGTGTGTGTGGTGTG	230-250	52
G205	(GT)7gcgtgtgcctgcgtctctgcgagtgcgtgc(GT)6	RP-CGACACGTACGCAACGAC	230-250	52
G206	(GT)8	FP-AAACTGGCCCTGCATTTTC	190-210	52
G206	(GT)8	RP-GGTCATGGCAATTTGAGACA	190-210	52
G209	(GT)8	FP-TTTGCACGTGTCCTGTGTTT	240-260	52
G209	(GT)8	RP-ACGACGACCACACACCACTA	240-260	52
G211	(GT)9	FP-ATGGCGTCGTATGTGTGTGT	200-210	52
G211	(GT)9	RP-GTTACGGCCGAATCAACAAC	200-210	52
G219	(GTT)10	FP-CCAGTTGTGCCGAACACAT	130-160	52
G219	(GTT)10	RP-CCAACAGCAGATTGCCAGTA	130-160	52
G225	(GTT)7	FP-GGGCAGTGGACCAGTTAGAG	250-270	52
G225	(GTT)7	RP-CCGAGGGAATAAACGACAAA	250-270	52
G228	(T)10	FP-CCTACGGACATGCCTGTTTT	280-310	52
G228	(T)10	RP-GCGGTAGGGGAAAAACAACT	280-310	52
G233	(TC)20	FP-CGTTCGTCCTTCTCCTCCTA	120-140	52
G233	(TC)20	RP-AGACGACTACGGACGACGAC	120-140	52
G234	(TC)7	FP-GTTGGGTTTGGCATTGAACT	190-210	52
G234	(TC)7	RP-GAAGGGGCGAACAAATAAAA	190-210	52
G244	(TG)6	FP-CAATCCGAAAATCACCACCT	230-250	52
G244	(TG)6	RP-GCACTCACATGCACACAAAC	230-250	52
G245	(TG)6	FP-CGTTGGTTGTTAGTCGGTCA	240-260	52
G245	(TG)6	RP-GAACGAAACAACGACGACAA	240-260	52
G249	(TG)6c(GT)7	FP-TATGTGTGCAACGGCAACTT	140-160	52
G249	(TG)6c(GT)7	RP-GCACACCCACCACACAATAG	140-160	52
G254	(TG)7	FP-TGAGTGCGTACGTGTGTCTG	100-120	52
G254	(TG)7	RP-GCGCGTGTTCACACATAGAC	100-120	52
G262	(TGGT)5	FP-TGTGCGTGTGTGTGTTTTTG	300-320	52
G262	(TGGT)5	RP-ACCACAACCCCTACCCTACC	300-320	52
G268	(TGT)5tattn(TTG)6	FP-TTGTTTGTTGTTGTTGTGTCTTG	290-305	52
G268	(TGT)5tattn(TTG)6	RP-CTACAGTACAGACCCGCCACT	290-305	52
G269	(TGT)6	FP-ATGCTGTTGATGCGTCAGTT	220-240	52
G269	(TGT)6	RP-TGCAGCAACAACAAATAAGACA	220-240	52
G273	(TGT)7	FP- TTTTTGGTATTGTTGTTGTCGT	250-270	52
G273	(TGT)7	RP- CTGCAGCAATAACAGCATCAG	250-270	52
G284	(TTG)6	FP-TGTGTTGTGTTGTGCTGTATGTA	160-170	52
G284	(TTG)6	RP-GCAGCAACATTAAAACGAACAG	160-170	52
G285	(TTG)6	FP-TTTGTGCGGTTGATGTTGTT	190-200	52
G285	(TTG)6	RP-CTACGTCAGCCCGTCATACC	190-200	52

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