From: Allelic variation of soybean flower color gene W4 encoding dihydroflavonol 4-reductase 2
Purpose | Target | Forward primer (5′-3′) | Reverse primer (5′-3′) |
---|---|---|---|
cDNA cloning | DFR2 | AACCAAAACAACGAGAGAGA | CTTATCCCTGATATGAAAGC |
cDNA sequencing | TGCTAGACATCATGAAAGCA | TGTGAACAGCATATGTACCT | |
DFR2 | CACTGCTCTTTCACTAATCA | GATTAGTGAAAGAGCAGTGA | |
TACCCTGAGTATAATGTCCT | TTCACGCATGCTTTCATGAT | ||
Cloning of genomic fragment | Upstream fragment of DFR2 | ACGGTTTCTTCCATTCCATT | ACTTGATTTCAGCCATGGTA |
Downstream fragment of DFR2 | GTTCATCAATGCACATAGAC | CTTATCCCTGATATGAAAGC | |
Sequencing of genomic fragment | Upstream fragment of DFR2 | TACAAGTTGTCATCACGATC | GAAGCTTTGATGAAGCCATT |
TTTGGTGTACACTCGTATGT | CACAATTATATCATTGGGCA | ||
ATGTAACATGATGGTTCGTG | AACCACCATTGCTTAATACC | ||
Downstream fragment of DFR2 | CTTTTTCTCTGCAGGTTTCA | TAGTGGATGAATATGATTCT | |
AAGTACCATTCCAACATTAA | GATAGATGACAGTTGTTGTC | ||
TGTTGTGCTCTTTGGCATAT | ACCCTGAGTATAATGTCCTT | ||
CAPS analysis | DFR2 | ACGGTTTCTTCCATTCCATT | CAAATGCTTCACCTTCTTCA |
Cloning of 5′ upstream region | Upstream fragment of DFR2 | AGAGATATATAAGAAGTTAGGA | TATCACGAAATAGTTTTTGTAAT |
Downstream fragment of DFR2 | CCTTTACCATCTACAAGATAA | ATGATGTAATATTGGGAACCT | |
Sequencing of 5′ upstream region | DFR2 | GAAAAGAGAAATAGGTATTATA | GTTTAACTAATCAAACTAAATT |
Real-time PCR | DFR2 | CCAAGGACCCTGAGAATGAA | CAGAAGTCAACATCGCTCCA |
Actin | GTCCTTTCAGGAGGTACAACC | CCACATCTGCTGGAAGGTGC |