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Table 2 PCR primers used in this study

From: Allelic variation of soybean flower color gene W4 encoding dihydroflavonol 4-reductase 2

Purpose

Target

Forward primer (5′-3′)

Reverse primer (5′-3′)

cDNA cloning

DFR2

AACCAAAACAACGAGAGAGA

CTTATCCCTGATATGAAAGC

cDNA sequencing

 

TGCTAGACATCATGAAAGCA

TGTGAACAGCATATGTACCT

DFR2

CACTGCTCTTTCACTAATCA

GATTAGTGAAAGAGCAGTGA

 

TACCCTGAGTATAATGTCCT

TTCACGCATGCTTTCATGAT

Cloning of genomic fragment

Upstream fragment of DFR2

ACGGTTTCTTCCATTCCATT

ACTTGATTTCAGCCATGGTA

Downstream fragment of DFR2

GTTCATCAATGCACATAGAC

CTTATCCCTGATATGAAAGC

Sequencing of genomic fragment

Upstream fragment of DFR2

TACAAGTTGTCATCACGATC

GAAGCTTTGATGAAGCCATT

TTTGGTGTACACTCGTATGT

CACAATTATATCATTGGGCA

ATGTAACATGATGGTTCGTG

AACCACCATTGCTTAATACC

Downstream fragment of DFR2

CTTTTTCTCTGCAGGTTTCA

TAGTGGATGAATATGATTCT

AAGTACCATTCCAACATTAA

GATAGATGACAGTTGTTGTC

TGTTGTGCTCTTTGGCATAT

ACCCTGAGTATAATGTCCTT

CAPS analysis

DFR2

ACGGTTTCTTCCATTCCATT

CAAATGCTTCACCTTCTTCA

Cloning of 5′ upstream region

Upstream fragment of DFR2

AGAGATATATAAGAAGTTAGGA

TATCACGAAATAGTTTTTGTAAT

Downstream fragment of DFR2

CCTTTACCATCTACAAGATAA

ATGATGTAATATTGGGAACCT

Sequencing of 5′ upstream region

DFR2

GAAAAGAGAAATAGGTATTATA

GTTTAACTAATCAAACTAAATT

Real-time PCR

DFR2

CCAAGGACCCTGAGAATGAA

CAGAAGTCAACATCGCTCCA

Actin

GTCCTTTCAGGAGGTACAACC

CCACATCTGCTGGAAGGTGC