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Figure 3 | BMC Plant Biology

Figure 3

From: Co-overexpression of two Heat Shock Factors results in enhanced seed longevity and in synergistic effects on seedling tolerance to severe dehydration and oxidative stress

Figure 3

Unaltered vegetative thermotolerance in the 35S:A4a seedlings. (A) Representative results from three independent experiments (n = 39) performed with the three 35S:A4a sibling line pairs. (top left) The 35S:A4a1 seedlings did not survive direct exposure to 48°C for 2.5 h (HS). (bottom left) The sibling NT1 seedlings did not survive the same treatment. After an acclimation treatment for 3 h at 40°C (acc), both the 35S:A4a1 (top right) and the sibling NT1 seedlings (bottom right) acquire thermotolerance in a similar way and survive the 48°C treatment. Scale bar, 1 cm. (B) Western analyses of HSP and tagged-HaHSFA4a accumulation at normal growth temperatures in seedlings from the different line pairs. A sample from heat acclimated NT3 seedlings (acc) was used as positive control. Plastidial sHSP western detection (sHSP P). Rest of symbols as in Figure 2.

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BMC Plant Biology

ISSN: 1471-2229

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